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biotinylated universal antibody (horse anti-mouse/rabbit igg)  (Vector Laboratories)


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    Vector Laboratories biotinylated universal antibody (horse anti-mouse/rabbit igg)
    Biotinylated Universal Antibody (Horse Anti Mouse/Rabbit Igg), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 7660 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+horse+anti+mouse+igg/Biotinylated+Universal+Antibody+(Horse+Anti-Mouse%2FRabbit+IgG)/custom%40ba-1400%4042532140
    Average 96 stars, based on 7660 article reviews
    biotinylated universal antibody (horse anti-mouse/rabbit igg) - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Axonal Projections of Neurons in the Brainstem Mesopontine Tegmental Anesthesia Area (MPTA) That Effect Anesthesia, Enabling Pain-Free Surgery
    Article Snippet: .. These sections were incubated overnight in polyclonal mouse antiNeuN antibody (MilliporeSigma, Burlington, MA, USA, catalog #MAB377, 1:20,000, RT) followed by 1.5 h in biotinylated horse anti-mouse IgG (Vector Laboratories, Burlingame, CA, USA, cat. BA-2001, 1:1000; 37 ◦C). .. Neurons were then visualized using the ABC reaction (avidin-biotin conjugate) and diaminobenzidine (DAB) (Vectastain Elite ABC kit; Vector Laboratories).

    Article Title: Small heterodimer partner protects against osteoarthritis by inhibiting IKKβ/NF-κB-mediated matrix-degrading enzymes in chondrocytes.
    Article Snippet: .. The sections were incubated with biotinylated horse anti-mouse IgG or goat anti-rabbit IgG secondary antibody (Vector Laboratories, Burlingame, CA, USA) for 1 h, followed by incubation with avidin-biotinylated horseradish peroxidase (HRP) complex (Vectastain Elite ABC-HRP kit, PK-6101, Vector Laboratories) at room temperature for 30 min. .. The signal was developed with the 3,3′-diaminobenzidine (DAB) chromogen (DAB Peroxidase HRP Substrate Kit; SK-4100; Vector Laboratories), and cell nuclei were labelled with haematoxylin.

    Article Title: Gastrodia elata Blume extract suppresses lipid accumulation in high-fat diet-fed rats: a biochemical and histopathological evaluation
    Article Snippet: Next, the primary antibody SREBP-1 (sterol regulatory element binding protein-1, diluted 1:400, Santa Cruz Biotechnology, Dallas, TX, USA) was incubated overnight at 4 °C. .. After incubation, the slides were rinsed in PBS and incubated with biotinylated horse anti-mouse IgG (Vector Laboratories, CA, USA), diluted in PBS for 1 h at room temperature. .. Next, the sections were rinsed in PBS and incubated with avidin-biotin complex reagent (Vector Laboratories, CA, USA) for 30 min at room temperature.

    Article Title: Characterizing Evoked Movement Patterns and Connectivity in Sensorimotor Cortex After Sensory Loss in Squirrel Monkeys.
    Article Snippet: In brief, sections were incubated in a blocker of 0.1 M PBS, pH 7.2, with 0.5% Triton X-100 and 5% normal horse serum for 1 h at room temperature before incubation in primary antibodies (mouse anti-VGLUT2, 1:5000, Millipore) in the blocker for 40–48 h at 4◦C. .. After rinsing, the sections were incubated in the blocker containing biotinylated horse anti-mouse IgG (Vector Laboratories, Newark, CA, USA; 1:200) for 90 min at room temperature followed by avidin/biotinbased peroxidase “ABC” incubation (one drop each of reagent A and B per 7mL of 0.1M PB, pH 7.2; ABC kits, PK-6100, Vector) for 90 min at room temperature. ..

    Article Title: Low-intensity muscle contraction exercise reduces pain sensitivity by modulating peripheral pathology and spinal sensitization in end-stage knee osteoarthritis rats
    Article Snippet: .. The sections were blocked with 1% bovine serum albumin (BSA) containing 1% normal horse serum for 60 min. Next, the sections were incubated with a mouse anti-CD68 monoclonal antibody (Cat No. MCA341R, 1:200; Bio-Rad, Hercules, CA), rabbit anti-ITGAX/CD11c polyclonal antibody (Cat No. LS- B15988 , 1:2,000; LifeSpan Biosciences, Seattle, WA), and rabbit anti-mannose receptor polyclonal antibody (Cat No. ab64693, 1:3,000; Abcam, Cambridge, MA) for 30 min, followed by incubation with biotinylated horse anti-mouse IgG (BA-2000, 1:2,000; Vector Laboratories, Burlingame, CA) or biotinylated goat anti-rabbit IgG (H + L) (BA-1000; 1:3,000; Vector Laboratories). .. Each section was stained using an avidin-biotin complex method (Vectastain Elite ABC kit; Vector Laboratories) and then visualized with a metal-enhanced DAB substrate kit (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Gastrodia elata Blume extract suppresses lipid accumulation in high-fat diet-fed rats: a biochemical and histopathological evaluation.
    Article Snippet: Next, the primary antibody SREBP-1 (sterol regulatory element binding protein-1, diluted 1:400, Santa Cruz Biotechnology, Dallas, TX, USA) was incubated overnight at 4 °C. .. After incubation, the slides were rinsed in PBS and incubated with biotinylated horse anti-mouse IgG (Vector Laboratories, CA, USA), diluted in PBS for 1 h at room temperature. .. Next, the sections were rinsed in PBS and incubated with avidin-biotin complex reagent (Vector Laboratories, CA, USA) for 30 min at room temperature.

    Comparison:

    Article Title: Achieving cell-type specific transduction with adeno-associated viral vectors in pigeons
    Article Snippet: ms anti-NeuN 1:500 , Thermo Fisher Scientific Cat# MA5-33103, RRID: AB_2802653 , gt anti-mouse Alexa Fluor 488 1:250 , Thermo Fisher Scientific Cat# A-11001, RRID: AB_2534069. .. Comparison of AAV Serotypes for Anterograde and Retrograde Transport , ms anti-ChR2 1:1000 , Progen Cat#651180, RRID: AB_2892521 , biotinylated horse anti-mouse IgG 1:500 , Vector Laboratories Cat# BA-2000, RRID: AB_2313581. .. rb anti-RFP 1:1000 , Antibodies-Online Cat# ABIN129578, RRID: AB_10781500 , gt anti-rabbit Alexa Fluor 594 1:500 , Thermo Fisher Scientific Cat# A32740, RRID: AB_2762824.

    Bioprocessing:

    Article Title: Achieving cell-type specific transduction with adeno-associated viral vectors in pigeons
    Article Snippet: ms anti-NeuN 1:500 , Thermo Fisher Scientific Cat# MA5-33103, RRID: AB_2802653 , gt anti-mouse Alexa Fluor 488 1:250 , Thermo Fisher Scientific Cat# A-11001, RRID: AB_2534069. .. Comparison of AAV Serotypes for Anterograde and Retrograde Transport , ms anti-ChR2 1:1000 , Progen Cat#651180, RRID: AB_2892521 , biotinylated horse anti-mouse IgG 1:500 , Vector Laboratories Cat# BA-2000, RRID: AB_2313581. .. rb anti-RFP 1:1000 , Antibodies-Online Cat# ABIN129578, RRID: AB_10781500 , gt anti-rabbit Alexa Fluor 594 1:500 , Thermo Fisher Scientific Cat# A32740, RRID: AB_2762824.

    Avidin-Biotin Assay:

    Article Title: Small heterodimer partner protects against osteoarthritis by inhibiting IKKβ/NF-κB-mediated matrix-degrading enzymes in chondrocytes.
    Article Snippet: .. The sections were incubated with biotinylated horse anti-mouse IgG or goat anti-rabbit IgG secondary antibody (Vector Laboratories, Burlingame, CA, USA) for 1 h, followed by incubation with avidin-biotinylated horseradish peroxidase (HRP) complex (Vectastain Elite ABC-HRP kit, PK-6101, Vector Laboratories) at room temperature for 30 min. .. The signal was developed with the 3,3′-diaminobenzidine (DAB) chromogen (DAB Peroxidase HRP Substrate Kit; SK-4100; Vector Laboratories), and cell nuclei were labelled with haematoxylin.

    Article Title: Characterizing Evoked Movement Patterns and Connectivity in Sensorimotor Cortex After Sensory Loss in Squirrel Monkeys.
    Article Snippet: In brief, sections were incubated in a blocker of 0.1 M PBS, pH 7.2, with 0.5% Triton X-100 and 5% normal horse serum for 1 h at room temperature before incubation in primary antibodies (mouse anti-VGLUT2, 1:5000, Millipore) in the blocker for 40–48 h at 4◦C. .. After rinsing, the sections were incubated in the blocker containing biotinylated horse anti-mouse IgG (Vector Laboratories, Newark, CA, USA; 1:200) for 90 min at room temperature followed by avidin/biotinbased peroxidase “ABC” incubation (one drop each of reagent A and B per 7mL of 0.1M PB, pH 7.2; ABC kits, PK-6100, Vector) for 90 min at room temperature. ..



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